Genetic and functional analysis of the basic replicon of pPS10, a plasmid specific for Pseudomonas isolated from Pseudomonas syringae patovar savastanoi

J Mol Biol. 1992 Jan 20;223(2):415-26. doi: 10.1016/0022-2836(92)90661-3.


The sequence of a 1823 base-pair region containing the replication functions of pPS10, a narrow host-range plasmid isolated from a strain of Pseudomonas savastanoi, is reported. The origin of replication, oriV, or pPS10 is contained in a 535 base-pair fragment of this sequence that can replicate in the presence of trans-acting function(s) of the plasmid. oriV contains four iterons of 22 base-pairs that are preceded by G+C-rich and A+T-rich regions. A dnaA box located adjacent to the repeats of the origin is dispensable but required for efficient replication of pPS10; A and T are equivalent bases at the 5' end of the box. repA, the gene of a trans-acting replication protein of 26,700 Mr has been identified by genetic and functional analysis. repA is adjacent to the origin of replication and is preceded by the consensus sequences of a typical sigma 70 promoter of Escherichia coli. The RepA protein has been identified, using the minicell system of E. coli, as a polypeptide with an apparent molecular mass of 26,000. A minimal pPS10 replicon has been defined to a continuous 1267 base-pair region of pPS10 that includes the oriV and repA sequences.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Bacterial Proteins
  • Base Sequence
  • DNA Helicases*
  • DNA Replication / genetics
  • DNA-Binding Proteins
  • Gene Expression Regulation
  • Molecular Sequence Data
  • Mutation
  • Plasmids / genetics*
  • Protein Conformation
  • Proteins*
  • Pseudomonas / genetics*
  • Reading Frames
  • Recombinant Fusion Proteins
  • Regulatory Sequences, Nucleic Acid / genetics
  • Replicon / genetics*
  • Trans-Activators*


  • Bacterial Proteins
  • DNA-Binding Proteins
  • DnaA protein, Bacteria
  • Proteins
  • Recombinant Fusion Proteins
  • Trans-Activators
  • replication initiator protein
  • DNA Helicases