Detection and measurement of neurofibromatosis-1 mouse optic glioma in vivo

Neuroimage. 2007 May 1;35(4):1434-7. doi: 10.1016/j.neuroimage.2007.02.019. Epub 2007 Feb 23.


One of the major limitations to preclinical mouse therapeutic evaluation is the inherent difficulty in imaging small tumors in vivo. We present a rapid and reliable method to detect optic glioma (OPG) in a mouse neurofibromatosis-1 model (Nf1(flox/mut)GFAP-Cre mice) in vivo using Manganese-Enhanced Magnetic Resonance Imaging (MEMRI). In a blinded study, 23 mice were chosen randomly from a cohort of Nf1(flox/mut)GFAP-Cre mice and two sets of age-matched controls. In all cases, OPG presence or absence was correctly identified. In addition, the OPG size and shape were accurately measured in vivo, facilitating the use of this model for preclinical drug studies.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Genes, Neurofibromatosis 1 / physiology
  • Glial Fibrillary Acidic Protein / genetics
  • Glial Fibrillary Acidic Protein / metabolism
  • Image Processing, Computer-Assisted
  • Magnetic Resonance Imaging
  • Manganese
  • Mice
  • Mice, Inbred C57BL
  • Mice, Transgenic
  • Neurofibromatosis 1 / pathology*
  • Optic Nerve Glioma / pathology*
  • Optic Nerve Neoplasms / pathology*


  • Glial Fibrillary Acidic Protein
  • Manganese