Immobilization of cholesterol esterase and cholesterol oxidase onto sol-gel films for application to cholesterol biosensor

Suman Singh; Rahul Singhal; B D Malhotra

doi:10.1016/j.aca.2006.09.010

Immobilization of cholesterol esterase and cholesterol oxidase onto sol-gel films for application to cholesterol biosensor

Anal Chim Acta. 2007 Jan 23;582(2):335-43. doi: 10.1016/j.aca.2006.09.010. Epub 2006 Sep 16.

Authors

Suman Singh¹, Rahul Singhal, B D Malhotra

Affiliation

¹ Central Mechanical Engineering Research Institute, G. Avenue, Durgapur 713209, West Bengal, India.

PMID: 17386511
DOI: 10.1016/j.aca.2006.09.010

Abstract

Cholesterol oxidase (ChOx) and cholesterol esterase (ChEt) have been covalently immobilized onto tetraethylorthosilicate (TEOS) sol-gel films. The tetraethylorthosilicate sol-gel/ChEt/ChOx enzyme films thus prepared have been characterized using scanning electron microscopic (SEM), UV-vis spectroscopic, Fourier-transform-infrared (FTIR) spectroscopic and amperometric techniques, respectively. The results of photometric measurements carried out on tetraethylorthosilicate sol-gel/ChEt/ChOx reveal thermal stability up to 55 degrees C, response time as 180 s, linearity up to 780 mg dL(-1) (12 mM), shelf life of 1 month, detection limit of 12 mg dL(-1) and sensitivity as 5.4 x 10(-5) Abs. mg(-1) dL(-1).

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Biosensing Techniques*
Cholesterol / analysis*
Cholesterol Oxidase / chemistry*
Enzymes, Immobilized / chemistry*
Sensitivity and Specificity
Spectroscopy, Fourier Transform Infrared
Sterol Esterase / chemistry*

Substances

Enzymes, Immobilized
Cholesterol
Cholesterol Oxidase
Sterol Esterase