Mass spectrometric detection of and similarities between 1-androgens

Anal Chim Acta. 2007 Mar 14;586(1-2):57-72. doi: 10.1016/j.aca.2006.10.058. Epub 2006 Nov 11.


Regularly new anabolic steroids appear on the black market. In most cases these substances are marketed on websites or are confiscated during inspections. 1,(5alpha)-Androstene-17beta-ol-3-one, also known as 1-testosterone, is one of these substances presented to body-builders as a nutritional supplement or a pro-hormone. 1-Testosterone closely resembles the natural hormone testosterone except for a 1,2-double bound instead of a 4,5-double bound. 1-Androstene-3beta,17beta-diol is transformed into 1-testosterone after oral administration. 1-Testosterone, 1-androstene-3beta,17beta-diol and some other related 'new' anabolic steroids were studied with gas chromatography coupled to mass spectrometry (GC-MS) and Liquid chromatography coupled to tandem mass spectrometry (LC-MS2) methods. Similarities in spectra to known analytes, which may lead to pitfalls in the interpretation of the derivatised analytes, are discussed.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Administration, Oral
  • Anabolic Agents / analysis*
  • Androgens / analysis*
  • Androstenediol / analysis
  • Chromatography, Gas / methods*
  • Chromatography, High Pressure Liquid / methods
  • Chromatography, Liquid / methods*
  • Doping in Sports
  • Gas Chromatography-Mass Spectrometry
  • Mass Spectrometry / methods*
  • Models, Chemical
  • Steroids / analysis*
  • Substance Abuse Detection / methods
  • Testosterone / analogs & derivatives*
  • Testosterone / analysis*
  • Testosterone / chemistry
  • Weight Lifting


  • Anabolic Agents
  • Androgens
  • Steroids
  • Testosterone
  • Androstenediol
  • 1-testosterone