Currently the northern provinces of Iran (Mazandaran, Golestan and Gilan) are the main tobacco growing regions in the country and this crop has an special importance in the national economy. Three tobacco types including flue-cured (mainly Coker 347 and some Virginia E1), burley (Burley 21) and oriental (Basma 178-2) are presently grown in these regions. Epidemics of viral diseases have occurred during the recent years in many tobacco fields in these areas. The quality of tobacco products which is much important, is adversely affected by plant pathogens specially viruses. In a survey on the viruses of tobacco, the fields in these regions were inspected and leaf samples from symptomatic plants were collected. Some plants had one or more of the symptoms such as dentate leaf margin, thicker leaf tissue and necrotic areas on the stem. The samples were tested for TSV infection by the DAS-ELISA method (Clark and Adams, 1977) using polyclonal antibody (AS-0615, DSMZ, Germany). TSV was detected in more than 79% of all tobacco samples from these three provinces. The TSV infection level among the tested samples was 86.8% in Gilan (Rasht, Bazar-Jomeh and Talesh), 82.3% in Mazandaran (Behshahr, Sari, Neka and Sourak) and 71.8% in Golestan (Gorgan, Aliabad and Minoodasht). No significant difference was seen among the infection levels for the mentioned commercial varieties and also some other tested varieties such as C176, K326 and MN944. It seems that there is no resistance sources against this virus within these varieties. Also the results of tests for TSV were similar in two consecutive years (2004 and 2005). It should be added that not all of the TSV infected plants showed the stated symptom types. Many of the TSV infected samples had mixed infections with one or more other viruses such as TSWV, CMV, PVY and TMV and there was almost no sample with a single TSV infection. This is the first report on the occurrence and distribution of TSV in the tobacco fields of Iran, too.