Virus neutralizing antibody response in mice and dogs with a bicistronic DNA vaccine encoding rabies virus glycoprotein and canine parvovirus VP2

Vaccine. 2007 May 16;25(20):4020-8. doi: 10.1016/j.vaccine.2007.02.051. Epub 2007 Mar 15.


A bicistronic DNA vaccine against rabies and parvovirus infection of dogs was developed by subcloning rabies glycoprotein and canine parvovirus (CPV) VP2 genes into a bicistronic vector. After characterizing the expression of both the proteins in vitro, the bicistronic DNA vaccine was injected in mice and induced immune response was compared with monocistronic DNA vaccines. There was no significant difference in ELISA and virus neutralizing (VN) antibody responses against rabies and CPV in mice immunized with either bicistronic or monocistronic DNA vaccine. Further, there was significantly similar protection in mice immunized with either bicistronic or monocistronic rabies DNA vaccine on rabies virus challenge. Similarly, dogs immunized with monocistronic and bicistronic DNA vaccines developed comparable VN antibodies against rabies and CPV. This study indicated that bicistronic DNA vaccine can be used in dogs to induce virus neutralizing immune responses against both rabies and CPV.

MeSH terms

  • Animals
  • Antibodies, Viral / biosynthesis
  • Antibodies, Viral / immunology*
  • Antigens, Surface / genetics
  • Antigens, Surface / immunology
  • Capsid Proteins / genetics
  • Capsid Proteins / immunology
  • Cattle
  • Cell Line
  • Cricetinae
  • Dogs
  • Glycoproteins / genetics
  • Glycoproteins / immunology
  • Mice
  • Parvovirus, Canine / genetics
  • Parvovirus, Canine / immunology*
  • Rabies / immunology
  • Rabies / prevention & control*
  • Rabies / veterinary
  • Rabies Vaccines / genetics
  • Rabies Vaccines / immunology*
  • Rabies Vaccines / pharmacology
  • Rabies virus / genetics
  • Rabies virus / immunology*
  • Vaccines, DNA / genetics
  • Vaccines, DNA / immunology*
  • Vaccines, DNA / pharmacology
  • Viral Proteins / genetics
  • Viral Proteins / immunology*


  • Antibodies, Viral
  • Antigens, Surface
  • Capsid Proteins
  • Glycoproteins
  • Rabies Vaccines
  • Vaccines, DNA
  • Viral Proteins