Abstract
We have developed an indirect enzyme-linked immunosorbent assay for detection of anti-dengue virus (DENV) immunoglobulin G antibodies using four recombinant DENV envelope polypeptides as antigens, which demonstrated a sensitivity of 89.4% and a specificity of 93.3%. These easily produced antigens are a feasible, cost-effective alternative for generating reagents for dengue serological tests.
Publication types
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Clinical Trial
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Antibodies, Viral / blood*
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Antigens, Viral / immunology*
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Dengue / blood
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Dengue / diagnosis*
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Dengue / immunology
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Enzyme-Linked Immunosorbent Assay / methods*
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Humans
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Immunoglobulin G / blood
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Immunoglobulin M / blood
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Recombinant Proteins / immunology*
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Sensitivity and Specificity
Substances
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Antibodies, Viral
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Antigens, Viral
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Immunoglobulin G
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Immunoglobulin M
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Recombinant Proteins