Characterization of A7r5 cell line transfected in a stable form by hSSAO/VAP-1 gene (A7r5 hSSAO/VAP-1 cell line)

J Neural Transm (Vienna). 2007;114(6):763-7. doi: 10.1007/s00702-007-0685-9. Epub 2007 Mar 29.


A smooth muscle cell line (A7r5) was stably transfected with the human SSAO/VAP-1 (hSSAO/VAP-1) gene. The expressed protein was located solely in the membrane fraction of the cell. However it was also shown to be released into the cell-culture medium. Both the membrane-bound and released, soluble, forms had SSAO enzyme activity. Although MAO-A is present in wild-type A7r5 cells, it was undetectable in the transfected cells.

MeSH terms

  • Amine Oxidase (Copper-Containing) / genetics*
  • Amine Oxidase (Copper-Containing) / metabolism*
  • Animals
  • Cell Adhesion Molecules / genetics*
  • Cell Adhesion Molecules / metabolism*
  • Cell Compartmentation / genetics
  • Cell Line
  • Cell Membrane / metabolism
  • Culture Media / pharmacology
  • Humans
  • Models, Biological
  • Muscle, Smooth, Vascular / metabolism*
  • Rats
  • Transfection / methods


  • Cell Adhesion Molecules
  • Culture Media
  • AOC3 protein, human
  • Amine Oxidase (Copper-Containing)