Characterization of A7r5 cell line transfected in a stable form by hSSAO/VAP-1 gene (A7r5 hSSAO/VAP-1 cell line)

M Solé; M Hernandez; M Boada; M Unzeta

doi:10.1007/s00702-007-0685-9

Characterization of A7r5 cell line transfected in a stable form by hSSAO/VAP-1 gene (A7r5 hSSAO/VAP-1 cell line)

J Neural Transm (Vienna). 2007;114(6):763-7. doi: 10.1007/s00702-007-0685-9. Epub 2007 Mar 29.

Authors

M Solé¹, M Hernandez, M Boada, M Unzeta

Affiliation

¹ Departament de Bioquímica i Biologia Molecular, Facultat de Medicina, Institut de Neurociències, Universitat Autònoma de Barcelona, Bellaterra, Barcelona, Spain.

PMID: 17393062
DOI: 10.1007/s00702-007-0685-9

Abstract

A smooth muscle cell line (A7r5) was stably transfected with the human SSAO/VAP-1 (hSSAO/VAP-1) gene. The expressed protein was located solely in the membrane fraction of the cell. However it was also shown to be released into the cell-culture medium. Both the membrane-bound and released, soluble, forms had SSAO enzyme activity. Although MAO-A is present in wild-type A7r5 cells, it was undetectable in the transfected cells.

MeSH terms

Amine Oxidase (Copper-Containing) / genetics*
Amine Oxidase (Copper-Containing) / metabolism*
Animals
Cell Adhesion Molecules / genetics*
Cell Adhesion Molecules / metabolism*
Cell Compartmentation / genetics
Cell Line
Cell Membrane / metabolism
Culture Media / pharmacology
Humans
Models, Biological
Muscle, Smooth, Vascular / metabolism*
Rats
Transfection / methods

Substances

Cell Adhesion Molecules
Culture Media
AOC3 protein, human
Amine Oxidase (Copper-Containing)