Female-specific DNA sequences in the chicken genome

J Hered. 2007 May-Jun;98(3):238-42. doi: 10.1093/jhered/esm010. Epub 2007 Mar 29.


Eight in silico W-specific sequences from the WASHUC1 chicken genome assembly gave female-specific PCR products using chicken DNA. Some of these fragments gave female-specific products with turkey and peacock DNA. Sequence analysis of these 8 fragments (3077 bp total) failed to detect any polymorphisms among 10 divergent chickens. In contrast, comparison of the DNA sequences of chicken with those of turkey and peacock revealed a nucleotide difference every 25 and 28 bp, respectively. Radiation hybrid mapping verified that these amplicons exist only on chromosome W. The homology of 6 W-specific fragments with chromo-helicase-DNA-binding gene and expressed sequenced tags from chicken and other species indicate that these fragments may have or have had a biological function. These fragments may be used for early sexing in commercial chicken and turkey flocks.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Chickens / genetics*
  • Chromosome Mapping
  • DNA / genetics*
  • Female
  • Genome*
  • Hybrid Cells
  • Polymerase Chain Reaction
  • Polymorphism, Single Nucleotide


  • DNA