Signals from chloroplasts converge to regulate nuclear gene expression

Science. 2007 May 4;316(5825):715-9. Epub 2007 Mar 29.

Abstract

Plastid-to-nucleus retrograde signaling coordinates nuclear gene expression with chloroplast function and is essential for the photoautotrophic life-style of plants. Three retrograde signals have been described, but little is known of their signaling pathways. We show here that GUN1, a chloroplast-localized pentatricopeptide-repeat protein, and ABI4, an Apetala 2 (AP2)-type transcription factor, are common to all three pathways. ABI4 binds the promoter of a retrograde-regulated gene through a conserved motif found in close proximity to a light-regulatory element. We propose a model in which multiple indicators of aberrant plastid function in Arabidopsis are integrated upstream of GUN1 within plastids, which leads to ABI4-mediated repression of nuclear-encoded genes.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Abscisic Acid
  • Amino Acid Motifs
  • Amino Acid Sequence
  • Arabidopsis / genetics
  • Arabidopsis / metabolism*
  • Arabidopsis Proteins / chemistry
  • Arabidopsis Proteins / genetics
  • Arabidopsis Proteins / metabolism*
  • Cell Nucleus / metabolism*
  • Cell Nucleus / microbiology*
  • Chloroplasts / metabolism*
  • DNA, Plant / metabolism
  • DNA-Binding Proteins / chemistry
  • DNA-Binding Proteins / genetics
  • DNA-Binding Proteins / metabolism*
  • Electron Transport
  • Gene Expression Regulation, Plant*
  • Light-Harvesting Protein Complexes / genetics
  • Lincomycin / pharmacology
  • Models, Biological
  • Molecular Sequence Data
  • Oligonucleotide Array Sequence Analysis
  • Plants, Genetically Modified
  • Promoter Regions, Genetic
  • Protoporphyrins / metabolism
  • Pyridazines / pharmacology
  • Signal Transduction
  • Transcription Factors / metabolism*

Substances

  • ABI4 protein, Arabidopsis
  • Arabidopsis Proteins
  • DNA, Plant
  • DNA-Binding Proteins
  • GUN1 protein, Arabidopsis
  • Light-Harvesting Protein Complexes
  • Protoporphyrins
  • Pyridazines
  • Transcription Factors
  • magnesium protoporphyrin
  • Abscisic Acid
  • Lincomycin
  • protoporphyrin IX
  • norflurazone