Protein composition of catalytically active human telomerase from immortal cells

Science. 2007 Mar 30;315(5820):1850-3. doi: 10.1126/science.1138596.


Telomerase is a ribonucleoprotein enzyme complex that adds 5'-TTAGGG-3' repeats onto the ends of human chromosomes, providing a telomere maintenance mechanism for approximately 90% of human cancers. We have purified human telomerase approximately 10(8)-fold, with the final elution dependent on the enzyme's ability to catalyze nucleotide addition onto a DNA oligonucleotide of telomeric sequence, thereby providing specificity for catalytically active telomerase. Mass spectrometric sequencing of the protein components and molecular size determination indicated an enzyme composition of two molecules each of telomerase reverse transcriptase, telomerase RNA, and dyskerin.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Cell Cycle Proteins / chemistry*
  • Cell Cycle Proteins / isolation & purification
  • Cell Line
  • Cell Line, Tumor
  • Centrifugation, Density Gradient
  • Humans
  • Molecular Sequence Data
  • Molecular Weight
  • Multienzyme Complexes / chemistry
  • Nuclear Proteins / chemistry*
  • Nuclear Proteins / isolation & purification
  • RNA / chemistry*
  • RNA / isolation & purification
  • Tandem Mass Spectrometry
  • Telomerase / chemistry*
  • Telomerase / isolation & purification
  • Telomerase / metabolism


  • Cell Cycle Proteins
  • DKC1 protein, human
  • Multienzyme Complexes
  • Nuclear Proteins
  • telomerase RNA
  • RNA
  • Telomerase

Associated data

  • GENBANK/CAA11970
  • GENBANK/O14746