Vitamin D and the vitamin D receptor are critical for control of the innate immune response to colonic injury

BMC Immunol. 2007 Mar 30:8:5. doi: 10.1186/1471-2172-8-5.

Abstract

Background: The active form of vitamin D (1,25(OH)2D3) has been shown to inhibit development of inflammatory bowel disease (IBD) in IL-10 KO mice. Here, the role of the vitamin D receptor (VDR) and 1,25(OH)2D3 in acute experimental IBD was probed.

Results: VDR KO mice were extremely sensitive to dextran sodium sulfate (DSS) and there was increased mortality of the VDR KO mice at doses of DSS that only caused a mild form of colitis in wildtype (WT) mice. DSS colitis in the VDR KO mice was accompanied by high colonic expression of TNF-alpha, IL-1 alpha, IL-1beta, IL-12, IFN-gamma, IL-10, MIP-1alpha and KC. DSS concentrations as low as 0.5% were enough to induce bleeding, ulceration and weight loss in VDR KO mice. VDR KO mice failed to recover following the removal of DSS, while WT mice showed signs of recovery within 5 days of DSS removal. The early mortality of DSS treated VDR KO mice was likely due to perforation of the bowel and resulting endotoxemia. VDR KO mice were hyper-responsive to exogenously injected LPS and cultures of the peritoneal exudates of moribund DSS treated VDR KO mice were positive for bacterial growth. 1,25(OH)2D3 in the diet or rectally decreased the severity and extent of DSS-induced inflammation in WT mice.

Conclusion: The data point to a critical role for the VDR and 1,25(OH)2D3 in control of innate immunity and the response of the colon to chemical injury.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acute Disease
  • Animals
  • Body Weight / drug effects
  • Calcitriol / metabolism*
  • Chemokine CCL3
  • Chemokine CCL4
  • Chemokines / biosynthesis
  • Chemokines / blood
  • Colitis / chemically induced
  • Colitis / immunology*
  • Colitis / mortality
  • Colitis / pathology
  • Dextran Sulfate
  • Immunity, Innate*
  • Inflammatory Bowel Diseases / immunology*
  • Inflammatory Bowel Diseases / pathology
  • Inflammatory Bowel Diseases / physiopathology
  • Interferon-gamma / biosynthesis
  • Interferon-gamma / blood
  • Interleukin-10 / biosynthesis
  • Interleukin-10 / blood
  • Interleukin-12 / biosynthesis
  • Interleukin-12 / blood
  • Interleukin-1alpha / biosynthesis
  • Interleukin-1alpha / blood
  • Interleukin-1beta / biosynthesis
  • Interleukin-1beta / blood
  • Macrophage Inflammatory Proteins / biosynthesis
  • Macrophage Inflammatory Proteins / blood
  • Mice
  • Mice, Knockout
  • Receptors, Calcitriol / genetics
  • Receptors, Calcitriol / metabolism*
  • Tumor Necrosis Factor-alpha / biosynthesis
  • Tumor Necrosis Factor-alpha / blood

Substances

  • Chemokine CCL3
  • Chemokine CCL4
  • Chemokines
  • Interleukin-1alpha
  • Interleukin-1beta
  • Macrophage Inflammatory Proteins
  • Receptors, Calcitriol
  • Tumor Necrosis Factor-alpha
  • Interleukin-10
  • keratinocyte-derived chemokines
  • Interleukin-12
  • Interferon-gamma
  • Dextran Sulfate
  • Calcitriol