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. 2007 Apr;1774(4):450-5.
doi: 10.1016/j.bbapap.2007.02.009. Epub 2007 Mar 6.

Mutational Analysis of O-acetylserine (Thiol) Lyase Conducted in Yeast Two-Hybrid System

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Mutational Analysis of O-acetylserine (Thiol) Lyase Conducted in Yeast Two-Hybrid System

Frantz Liszewska et al. Biochim Biophys Acta. .

Abstract

Cysteine biosynthesis, achieved by the sequential reaction of two enzymes, serine acetyltransferase and O-acetylserine (thiol) lyase (OASTL), represents the final step of sulfur assimilation pathway in plants and bacteria. The two enzymes form a bi-enzymatic cysteine synthase complex through specific protein-protein interactions. To identify the amino acids important for cysteine synthase complex formation, several mutations in bacterial OASTL were designed. Effects of mutagenesis were verified in a yeast two-hybrid model that allowed monitoring both, protein-protein interactions and the enzymatic activity of OASTL.

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