Multiple Extracellular Activities in Drosophila Egg Perivitelline Fluid Are Required for Establishment of Embryonic Dorsal-Ventral Polarity

Cell. 1992 Feb 7;68(3):429-40. doi: 10.1016/0092-8674(92)90181-b.

Abstract

Twelve maternal effect genes (the dorsal group and cactus) are required for the establishment of the embryonic dorsal-ventral axis in the Drosophila embryo. Embryonic dorsal-ventral polarity is defined within the perivitelline compartment surrounding the embryo by the ventral formation of a ligand for the Toll receptor. Here, by transplantation of perivitelline fluid we demonstrate the presence of three separate activities present in the perivitelline fluid that can restore dorsal-ventral polarity to mutant easter, snake, and spätzle embryos, respectively. These activities are not capable of defining the polarity of the dorsal-ventral axis; instead they restore structures according to the intrinsic dorsal-ventral polarity of the mutant embryos. They appear to be involved in the ventral formation of a ligand for the Toll protein. This process requires serine proteolytic activity; the injection of serine protease inhibitors into the perivitelline space of wild-type embryos results in the formation of dorsalized embryos.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Biological Assay
  • Cell Polarity
  • Drosophila / embryology*
  • Drosophila / genetics
  • Extracellular Space / metabolism
  • Morphogenesis / genetics
  • Vitelline Membrane / metabolism