Construction of mouse adenovirus type 1 mutants

Methods Mol Med. 2007;130:41-59. doi: 10.1385/1-59745-166-5:41.

Abstract

Mouse adenovirus provides a model for studying adenovirus pathogenesis in the natural host. The ability to make viral mutants allows the investigation of specific mouse adenoviral gene contributions to virus-host interactions. Methods for propagation and titration of wild-type mouse adenovirus, production of viral DNA and viral DNA-protein complex, and transfection of mouse cells to obtain mouse adenovirus mutants are described in this chapter. Plaque purification, propagation, and titration of the mutant viruses are also presented.

MeSH terms

  • Adenoviridae / genetics*
  • Adenoviridae / isolation & purification*
  • Animals
  • Centrifugation, Density Gradient
  • DNA / genetics
  • DNA, Viral / genetics
  • DNA, Viral / isolation & purification
  • Indicators and Reagents
  • Mice
  • Mutation
  • Transfection / methods
  • Viral Plaque Assay
  • Viral Proteins / genetics
  • Viral Proteins / isolation & purification

Substances

  • DNA, Viral
  • Indicators and Reagents
  • Viral Proteins
  • DNA