Capture ELISA quantitation of mouse adenovirus type 1 in infected organs

Methods Mol Med. 2007;130:215-21. doi: 10.1385/1-59745-166-5:215.

Abstract

A capture enzyme-linked immunosorbent assay (ELISA) was optimized for identification of mouse adenovirus type 1 in infected brain homogenates. The ELISA method allows for a much faster quantitation of virus in infected organs than plaque assays. Methods for organ homogenization and the subsequent capture ELISA are described in this chapter.

MeSH terms

  • Adenoviridae / isolation & purification*
  • Adenoviridae Infections / diagnosis*
  • Animals
  • Brain / virology*
  • Enzyme-Linked Immunosorbent Assay / methods
  • Indicators and Reagents
  • Mice
  • Safety
  • Sensitivity and Specificity
  • Viral Plaque Assay

Substances

  • Indicators and Reagents