Microtiter plate transformation using the LiAc/SS carrier DNA/PEG method

R Daniel Gietz; Robert H Schiestl

doi:10.1038/nprot.2007.16

Microtiter plate transformation using the LiAc/SS carrier DNA/PEG method

Nat Protoc. 2007;2(1):5-8. doi: 10.1038/nprot.2007.16.

Authors

R Daniel Gietz¹, Robert H Schiestl

Affiliation

¹ Department of Biochemistry and Medical Genetics, University of Manitoba, T250-770 Bannatyne Ave., Winnipeg, Manitoba R3E 0W3, Canada.

PMID: 17401331
DOI: 10.1038/nprot.2007.16

Abstract

Here, we describe a protocol that has been adapted for the transformation of yeast cells in 96-well microtiter plates. This protocol can be tailored for multiple applications and is suitable for high-throughput applications. It can be completed in 2-3 h, once the yeast cells have been grown depending on the heat shock used.

MeSH terms

Acetates
Cell Culture Techniques / instrumentation
Cell Culture Techniques / methods*
DNA, Single-Stranded / genetics
Hot Temperature
Polyethylene Glycols
Saccharomyces cerevisiae / cytology
Saccharomyces cerevisiae / genetics*
Saccharomyces cerevisiae / growth & development
Transformation, Genetic*

Substances

Acetates
DNA, Single-Stranded
Polyethylene Glycols
lithium acetate