High-efficiency yeast transformation using the LiAc/SS carrier DNA/PEG method

Nat Protoc. 2007;2(1):31-4. doi: 10.1038/nprot.2007.13.


Here we describe a high-efficiency version of the lithium acetate/single-stranded carrier DNA/PEG method of transformation of Saccharomyces cerevisiae. This method currently gives the highest efficiency and yield of transformants, although a faster protocol is available for small number of transformations. The procedure takes up to 1.5 h, depending on the length of heat shock, once the yeast culture has been grown. This method is useful for most transformation requirements.

MeSH terms

  • Acetates
  • DNA, Single-Stranded / genetics
  • Genetic Techniques*
  • Hot Temperature
  • Polyethylene Glycols
  • Saccharomyces cerevisiae / cytology
  • Saccharomyces cerevisiae / genetics*
  • Transformation, Genetic*


  • Acetates
  • DNA, Single-Stranded
  • Polyethylene Glycols
  • lithium acetate