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, 2 (1), 38-41

Large-scale High-Efficiency Yeast Transformation Using the LiAc/SS Carrier DNA/PEG Method

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Large-scale High-Efficiency Yeast Transformation Using the LiAc/SS Carrier DNA/PEG Method

R Daniel Gietz et al. Nat Protoc.

Abstract

Here, we describe a Library screen transformation protocol using the lithium acetate/single-stranded carrier DNA/PEG method of transformation for Saccharomyces cerevisiae. This method is suitable for screening complex plasmid libraries such as those used for yeast two-hybrid analysis. This procedure takes up to 2.5 h to complete once the yeast culture has been grown.

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