Large-scale high-efficiency yeast transformation using the LiAc/SS carrier DNA/PEG method

Nat Protoc. 2007;2(1):38-41. doi: 10.1038/nprot.2007.15.


Here, we describe a Library screen transformation protocol using the lithium acetate/single-stranded carrier DNA/PEG method of transformation for Saccharomyces cerevisiae. This method is suitable for screening complex plasmid libraries such as those used for yeast two-hybrid analysis. This procedure takes up to 2.5 h to complete once the yeast culture has been grown.

MeSH terms

  • Acetates
  • DNA, Single-Stranded / genetics
  • Gene Library
  • Genetic Techniques*
  • Hot Temperature
  • Plasmids
  • Polyethylene Glycols
  • Saccharomyces cerevisiae / cytology
  • Saccharomyces cerevisiae / genetics*
  • Transformation, Genetic*


  • Acetates
  • DNA, Single-Stranded
  • Polyethylene Glycols
  • lithium acetate