Quantification of F2-isoprostanes as a biomarker of oxidative stress

Nat Protoc. 2007;2(1):221-6. doi: 10.1038/nprot.2006.375.


Oxidant stress has been implicated in a wide variety of disease processes. One method to quantify oxidative injury is to measure lipid peroxidation. Quantification of a group of prostaglandin F(2alpha)-like compounds derived from the nonezymatic oxidation of arachidonic acid, termed the F2-isoprostanes (F2-IsoPs), provides an accurate assessment of oxidative stress both in vitro and in vivo. In fact, in a recent independent study sponsored by the National Institutes of Health (NIH), F2-IsoPs were shown to be the most reliable index of in vivo oxidant stress when compared against other well known biomarkers. This protocol details our laboratory's method to quantify F2-IsoPs in biological fluids and tissues using gas chromatography-mass spectrometry (GC-MS). This procedure can be completed for 12-15 samples in 6-8 h.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Biomarkers / analysis*
  • Chemistry Techniques, Analytical / methods*
  • F2-Isoprostanes / analysis*
  • F2-Isoprostanes / chemistry
  • Gas Chromatography-Mass Spectrometry / methods*
  • Humans
  • Oxidative Stress*


  • Biomarkers
  • F2-Isoprostanes