Wogonin, a plant flavone, potentiates etoposide-induced apoptosis in cancer cells

Ann N Y Acad Sci. 2007 Jan:1095:521-6. doi: 10.1196/annals.1397.056.

Abstract

Etoposide, a podophylotoxin anticancer agent, induces apoptotic cell death in normal and cancer cells. Etoposide-induced apoptosis plays a role in not only anticancer effect but also adverse reaction, such as myelosuppression. Since we have found that wogonin, a flavone found in Scutellaria baicalensis Georgi, prevents thymocyte apoptosis induced by various compounds including etoposide, we examined the effect of this flavone on etoposide-induced apoptosis in cancer cells. Although 100 muM wogonin itself significantly increased DNA fragmentation in HL-60 cells, this change was not observed in Jurkat cells. On the other hand, this flavone significantly potentiated etoposide-induced apoptosis in Jurkat and HL-60 cells. Similarly, wogonin accelerated etoposide-induced cell death in lung cancer cells. Since wogonin had no effect on the action of other anticancer agents, such as 5-FU and cisplatin, this flavone seems to accelerate only etoposide-induced apoptotic cell death in cancer cells. These results suggest that the modification of etoposide-induced apoptosis by wogonin may be available to reduce the adverse reaction of this agent.

MeSH terms

  • Animals
  • Antineoplastic Agents, Phytogenic / pharmacology*
  • Apoptosis / drug effects*
  • Drug Synergism
  • Etoposide / pharmacology*
  • Flavanones / pharmacology*
  • HL-60 Cells
  • Humans
  • Jurkat Cells
  • Leukemia, Promyelocytic, Acute / drug therapy
  • Leukemia, Promyelocytic, Acute / pathology*
  • Leukemia, T-Cell / drug therapy
  • Leukemia, T-Cell / pathology*
  • Rats
  • Scutellaria

Substances

  • Antineoplastic Agents, Phytogenic
  • Flavanones
  • Etoposide
  • wogonin