Nonradioactive detection of telomerase activity using the telomeric repeat amplification protocol

Nat Protoc. 2006;1(3):1583-90. doi: 10.1038/nprot.2006.239.


The telomeric repeat amplification protocol (TRAP) is a two-step process for analyzing telomerase activity in cell or tissue extracts. Recent modifications of this sensitive assay include elimination of radioactivity by using a fluorescently labeled primer instead of a radiolabeled primer. In addition, the TRAP assay has been modified for real-time, quantitative PCR analysis. Here, we describe cost-effective procedures for detection of telomerase activity using a fluorescent-based assay as well as by using real-time PCR. These modified TRAP assays can be accomplished within 4 h (from lysis of samples to analysis of telomerase products).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • DNA Primers
  • Fluorescence
  • Nucleic Acid Amplification Techniques / methods*
  • Telomerase / analysis*
  • Telomere / genetics*
  • Terminal Repeat Sequences / genetics


  • DNA Primers
  • Telomerase