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, 1 (6), 2856-60

In-gel Digestion for Mass Spectrometric Characterization of Proteins and Proteomes

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In-gel Digestion for Mass Spectrometric Characterization of Proteins and Proteomes

Andrej Shevchenko et al. Nat Protoc.

Abstract

In-gel digestion of proteins isolated by gel electrophoresis is a cornerstone of mass spectrometry (MS)-driven proteomics. The 10-year-old recipe by Shevchenko et al. has been optimized to increase the speed and sensitivity of analysis. The protocol is for the in-gel digestion of both silver and Coomassie-stained protein spots or bands and can be followed by MALDI-MS or LC-MS/MS analysis to identify proteins at sensitivities better than a few femtomoles of protein starting material.

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