[Allogenic islet transplantation on the rat liver after allogenic nonparenchymal cells injection in the thymus]

Arq Gastroenterol. 2006 Oct-Dec;43(4):321-7. doi: 10.1590/s0004-28032006000400015.
[Article in Portuguese]


Background: [corrected] The major indication for pancreas or islet transplantation is diabetes mellitus type I. This process has to supply the insulin necessity keeping glucose under control

Aim: We studied allogenic islet transplantation on the rat liver, Wistar (RT1u) to Lewis (RT1(1)) as a recipient. Control group (n = 8) and nonparenchymal cell group (n = 8) respectively with injection of Hanks solution and nonparenchymal cells in the thymus before islet transplantation.

Material and methods: With the method of isolation and purification of the islets we obtained both in the control group 3.637 +/-783,3 islets with purity of 85 +/- 3,52% and nonparenchymal cell group 3.270 +/- 770 islets with purity of 84,25 +/- 2,76%. The nonparenchymal cells were retrieved from the liver and we obtained 2 x 106 cells. Diabetes was induced by i.v. streptozotocin

Results: Control group the transplantation of 3.637 +/- 783,3 islets in the rat liver normalized glucose test, 7,21 +/- 0,57 mmol/L in the 2nd postoperative day. Acute rejection came in the 6th postoperative day with significantly increase of glucose test in nonparenchymal cell group, the transplantation of 3.270 +/- 770 islets in the rat liver, almost normalized the glucose test was 17,95 +/- 5,33 mmol/L in the 2nd postoperative day. From the 4th postoperative day to 10th postoperative day. The glucose test increase significantly showing an early acute rejection

Conclusion: The injection of nonparenchymal cells in the thymus before allogenic islet transplantation in the rat liver lead to an early acute rejection.

Publication types

  • Evaluation Study

MeSH terms

  • Animals
  • Blood Glucose / analysis
  • Cell Separation / methods
  • Diabetes Mellitus, Experimental / surgery*
  • Disease Models, Animal
  • Female
  • Graft Rejection / immunology*
  • Graft Survival
  • Immunosuppression Therapy / methods
  • Islets of Langerhans / cytology
  • Islets of Langerhans Transplantation / immunology
  • Islets of Langerhans Transplantation / methods*
  • Liver*
  • Male
  • Rats
  • Rats, Inbred Lew
  • Rats, Wistar
  • Streptozocin
  • Thymus Gland / immunology*


  • Blood Glucose
  • Streptozocin