Evaluation of polymerase chain reaction and culture for diagnosis of pertussis in the control of a county-wide outbreak focused among adolescents and adults

Clin Infect Dis. 2007 May 1;44(9):1216-9. doi: 10.1086/513432. Epub 2007 Mar 19.

Abstract

During a large pertussis outbreak, culture and polymerase chain reaction (PCR) were used to identify 149 case patients; of these case patients, 79 had positive PCR and culture results, 59 had positive PCR results and negative culture results, 11 had negative PCR results and positive culture results (10 PCR-negative, culture-positive specimens were collected < or = 14 days after illness onset). PCR and culture of samples obtained < or = 2 weeks after illness onset and PCR of samples obtained > 2 weeks after illness onset proved to be most diagnostically useful.

Publication types

  • Evaluation Study

MeSH terms

  • Adolescent
  • Adult
  • Child
  • Child, Preschool
  • Disease Outbreaks*
  • Humans
  • Infant
  • Infection Control / methods*
  • Microbiological Techniques / standards*
  • Middle Aged
  • Polymerase Chain Reaction / standards*
  • Time Factors
  • Whooping Cough / diagnosis*
  • Whooping Cough / epidemiology*
  • Wisconsin / epidemiology