Model for spiral columns and stationary phase retention in synchronous coil planet centrifuges

Y H Guan; D Fisher; I A Sutherland

doi:10.1016/j.chroma.2007.03.065

Model for spiral columns and stationary phase retention in synchronous coil planet centrifuges

J Chromatogr A. 2007 Jun 1;1151(1-2):136-41. doi: 10.1016/j.chroma.2007.03.065. Epub 2007 Mar 21.

Authors

Y H Guan¹, D Fisher, I A Sutherland

Affiliation

¹ Brunel Institute for Bioengineering, Brunel University West London, Uxbridge, Middlesex UB8 3PH, UK. hugh.guan@brunel.ac.uk

PMID: 17408676
DOI: 10.1016/j.chroma.2007.03.065

Abstract

Stationary phase retention in a synchronous coil planet centrifuge or high-speed counter-current chromatography (CCC) relies on the interplay of hydrostatic (tangential and normal centrifugal) and hydrodynamic (Archimedean screw and mobile phase drag) forces. By offering a set of quantitative or semi-quantitative theoretical frameworks, this work has resolved fundamental questions such as "in the absence of mobile phase flow, how is the distribution of the two phases in a CCC column determined?" and "for Type-J CCC, do the helical and the spiral columns lead to similar performance?"

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Countercurrent Distribution / instrumentation*
Countercurrent Distribution / methods*
Models, Theoretical*