Improved 16S rRNA-targeted probe set for analysis of sulfate-reducing bacteria by fluorescence in situ hybridization

J Microbiol Methods. 2007 Jun;69(3):523-8. doi: 10.1016/j.mimet.2007.02.009. Epub 2007 Feb 28.


An updated dataset of in silico specificities for 54 previously published 16S rRNA-targeted oligonucleotides was assembled to provide guidance for reliable fluorescence in situ hybridization (FISH) analysis of sulfate-reducing bacteria. Additionally, six new FISH probes were developed for major deltaproteobacterial taxa, including a probe trio targeting most Deltaproteobacteria and Gemmatimonadetes.

Publication types

  • Evaluation Study

MeSH terms

  • Databases, Nucleic Acid
  • Deltaproteobacteria / genetics
  • Deltaproteobacteria / isolation & purification
  • In Situ Hybridization, Fluorescence / methods*
  • Oligonucleotide Probes*
  • RNA, Ribosomal, 16S / genetics*
  • Sensitivity and Specificity
  • Species Specificity
  • Sulfates / metabolism*
  • Sulfur-Reducing Bacteria / genetics
  • Sulfur-Reducing Bacteria / isolation & purification*


  • Oligonucleotide Probes
  • RNA, Ribosomal, 16S
  • Sulfates