Abstract
Poliovirus RNA purified from virus-specific polyribosomes does not contain m7G in a 5'-5'-pyrophosphate linkage at its 5'-end. The only potential 5'-end found in ribonuclease digests of this RNA is pUp, which is present in a yield of 1 mole/mole of poliovirus RNA. We conclude that a 5'-terminal m7G is not required for translation of at least one RNA species in animal cells.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Base Sequence
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Electrophoresis, Paper / methods
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Poliovirus / analysis*
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Polyribosomes / analysis*
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RNA, Messenger / analysis*
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RNA, Messenger / isolation & purification
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RNA, Viral / analysis*
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RNA, Viral / isolation & purification
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Uracil Nucleotides / analysis*
Substances
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RNA, Messenger
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RNA, Viral
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Uracil Nucleotides