5'-terminal structure of poliovirus polyribosomal RNA is pUp

Proc Natl Acad Sci U S A. 1976 Feb;73(2):327-30. doi: 10.1073/pnas.73.2.327.

Abstract

Poliovirus RNA purified from virus-specific polyribosomes does not contain m7G in a 5'-5'-pyrophosphate linkage at its 5'-end. The only potential 5'-end found in ribonuclease digests of this RNA is pUp, which is present in a yield of 1 mole/mole of poliovirus RNA. We conclude that a 5'-terminal m7G is not required for translation of at least one RNA species in animal cells.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Base Sequence
  • Electrophoresis, Paper / methods
  • Poliovirus / analysis*
  • Polyribosomes / analysis*
  • RNA, Messenger / analysis*
  • RNA, Messenger / isolation & purification
  • RNA, Viral / analysis*
  • RNA, Viral / isolation & purification
  • Uracil Nucleotides / analysis*

Substances

  • RNA, Messenger
  • RNA, Viral
  • Uracil Nucleotides