Spheres isolated from 9L gliosarcoma rat cell line possess chemoresistant and aggressive cancer stem-like cells

Stem Cells. 2007 Jul;25(7):1645-53. doi: 10.1634/stemcells.2006-0624. Epub 2007 Apr 5.

Abstract

The rat 9L gliosarcoma is a widely used syngeneic rat brain tumor model that closely simulates glioblastoma multiforme when implanted in vivo. In this study, we sought to isolate and characterize a subgroup of cancer stem-like cells (CSLCs) from the 9L gliosarcoma cell line, which may represent the tumor-initiating subpopulation of cells. We demonstrate that these CSLCs form clonal-derived spheres in media devoid of serum supplemented with the mitogens epidermal growth factor and basic fibroblast growth factor, express the NSC markers Nestin and Sox2, self-renew, and differentiate into neuron-like and glial cells in vitro. More importantly, these cells can propagate and recapitulate tumors when implanted into the brain of syngeneic Fisher rats, and they display a more aggressive course compared with 9L gliosarcoma cells grown in monolayer cultures devoid of mitogens. Furthermore, we compare the chemosensitivity and proliferation rate of 9L gliosarcoma cells grown as a monolayer to those of cells grown as floating spheres and show that the sphere-generated cells have a lower proliferation rate, are more chemoresistant, and express several antiapoptosis and drug-related genes, which may prove to have important clinical implications. Disclosure of potential conflicts of interest is found at the end of this article.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Biomarkers, Tumor / metabolism
  • Cell Proliferation
  • Cell Separation
  • Drug Resistance, Neoplasm*
  • Gliosarcoma / pathology*
  • Luminescent Measurements
  • Neoplastic Stem Cells / drug effects
  • Neoplastic Stem Cells / pathology*
  • Neuroglia / metabolism
  • Neurons / metabolism
  • Rats
  • Spheroids, Cellular / drug effects
  • Spheroids, Cellular / pathology*
  • Survival Analysis
  • Tumor Cells, Cultured

Substances

  • Biomarkers, Tumor