Extraction of plant RNA

Methods Mol Biol. 2007:362:309-14. doi: 10.1007/978-1-59745-257-1_21.

Abstract

This protocol details an RNA preparation for medium-scale, high-purity RNA production from higher plants. It uses hot acid phenol with standard sodium acetate ethanol precipitation and is suitable for producing RNA for both Northern blotting and enzyme-based downstream applications such as RT-PCR and microarray studies.

MeSH terms

  • Arabidopsis / chemistry
  • Arabidopsis / genetics
  • Blotting, Northern
  • Chemical Precipitation
  • Ethanol
  • Oligonucleotide Array Sequence Analysis
  • Phenol
  • RNA, Plant / genetics
  • RNA, Plant / isolation & purification*
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sodium Acetate

Substances

  • RNA, Plant
  • Phenol
  • Ethanol
  • Sodium Acetate