Abstract
This protocol details an RNA preparation for medium-scale, high-purity RNA production from higher plants. It uses hot acid phenol with standard sodium acetate ethanol precipitation and is suitable for producing RNA for both Northern blotting and enzyme-based downstream applications such as RT-PCR and microarray studies.
MeSH terms
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Arabidopsis / chemistry
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Arabidopsis / genetics
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Blotting, Northern
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Chemical Precipitation
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Ethanol
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Oligonucleotide Array Sequence Analysis
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Phenol
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RNA, Plant / genetics
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RNA, Plant / isolation & purification*
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Reverse Transcriptase Polymerase Chain Reaction
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Sodium Acetate
Substances
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RNA, Plant
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Phenol
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Ethanol
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Sodium Acetate