Protein extraction, fractionation, and purification from cyanobacteria

Methods Mol Biol. 2007:362:365-73. doi: 10.1007/978-1-59745-257-1_26.

Abstract

This chapter deals with methods of protein extraction from cyanobacterial cells based on work in the circadian model organism Synechococcus elongatus PCC 7942. Some of these techniques have already been used successfully for analysis of circadian rhythms in cyanobacteria, whereas others are heretofore unpublished, but may yield exciting results in the near future.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Bacterial Proteins / isolation & purification*
  • Chromatography, Affinity
  • Circadian Rhythm
  • Cyanobacteria / chemistry*
  • Electrophoresis, Polyacrylamide Gel
  • Immunoprecipitation
  • Synechococcus / chemistry

Substances

  • Bacterial Proteins