Plant Protein Extraction

Methods Mol Biol. 2007;362:379-83. doi: 10.1007/978-1-59745-257-1_28.

Abstract

A method is presented for the extraction of total protein from Arabidopsis thaliana tissue. The protocol was designed for the solubilization of a range of proteins and their efficient and quantitative recovery. It is especially compatible with the small quantities of available tissue often associated with this species and was originally intended for Western blot preparations. Samples extracted using this method can be quantitated directly using a commercially available kit.

MeSH terms

  • Arabidopsis / chemistry
  • Arabidopsis Proteins / isolation & purification*
  • Blotting, Western
  • Sodium Dodecyl Sulfate
  • Solubility

Substances

  • Arabidopsis Proteins
  • Sodium Dodecyl Sulfate