Abstract
RNA in situ hybridization is a commonly used technique to achieve spatiotemporal detection of transcripts in tissues. This chapter gives an overview of novel techniques using fluorescent dyes, signal amplification methods, and confocal microscopy in regard to chronobiological applications on Drosophila adult brains.
MeSH terms
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Animals
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Antibodies
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Base Sequence
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Brain / physiology
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Chronobiology Phenomena / genetics*
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Chronobiology Phenomena / physiology*
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DNA / genetics
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Drosophila melanogaster / genetics*
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Drosophila melanogaster / physiology*
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Endopeptidase K
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Fluorescent Dyes
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Genes, Insect
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Genetic Vectors
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Haptens
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In Situ Hybridization / methods*
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In Situ Hybridization, Fluorescence / methods
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Microscopy, Confocal
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Molecular Sequence Data
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RNA / genetics*
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RNA Probes
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Transcription, Genetic
Substances
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Antibodies
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Fluorescent Dyes
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Haptens
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RNA Probes
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RNA
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DNA
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Endopeptidase K