Excretion profile of boldenone and its metabolites after oral administration to veal calves

Anal Chim Acta. 2007 Apr 25;589(2):269-74. doi: 10.1016/j.aca.2007.03.008. Epub 2007 Mar 12.


The residue profiles of boldenone (17beta-Bol), its epimer (17alpha-Bol) and the related compound androsta-1,4-diene-3,17-dione (ADD), were investigated by liquid chromatography-tandem mass spectrometry (LC-MS/MS) in urine of male calves orally treated with boldenone, boldenone esters, and/or ADD. In all the experiments with the administered steroids residues of 17alpha-Bol decreased rapidly after end of treatment; detectable amounts of 17alpha-Bol were however noticed along the withdrawal observation period after end of treatment. Differently, residues of 17beta-Bol were detectable only shortly after administration. This in vivo research concerning oral treatments of cattle with boldenone related substances proves ADD to be a very active boldenone precursor in bovine animals.

MeSH terms

  • Administration, Oral
  • Anabolic Agents / administration & dosage
  • Anabolic Agents / urine*
  • Animals
  • Cattle
  • Chromatography, Liquid / methods
  • Male
  • Tandem Mass Spectrometry / methods
  • Testosterone / administration & dosage
  • Testosterone / analogs & derivatives*
  • Testosterone / urine


  • Anabolic Agents
  • Testosterone
  • boldenone