Cross-neutralizing Antibodies in Rabbits Immunized With HIV-1 gp160 Purified From Simian Cells Infected With a Recombinant Vaccinia Virus

AIDS Res Hum Retroviruses. 1991 Oct;7(10):791-8. doi: 10.1089/aid.1991.7.791.

Abstract

A recombinant vaccinia virus in which the transcription of the human immunodeficiency virus type 1 (BRU isolate) env gene is driven by the 11K late vaccinia promoter yields about 10-fold higher amounts of gp160 env protein upon infection of monkey cells than does a recombinant in which gp160 is expressed using the 7.5K early-late promoter. The gp160 was purified from detergent lysates of infected cells by lentil lectin affinity chromatography followed by immunoaffinity chromatography, and was obtained in yields of 1-2 mg/10(9) cells of material estimated to be about 70% pure. Pairs of rabbits were immunized with purified gp160 using either one of five different adjuvants or an immunostimulating complex. In all cases a substantial humoral immune response was obtained after boosting, including an activity that neutralized the homologous (BRU) isolate of HIV-1. In some cases, this activity also neutralized two distantly related isolates, SF2 and MN.

MeSH terms

  • Animals
  • Cell Line
  • Cross Reactions
  • Gene Products, env / genetics
  • Gene Products, env / immunology*
  • Gene Products, env / isolation & purification
  • Genes, env
  • HIV Antibodies / biosynthesis*
  • HIV Envelope Protein gp160
  • HIV-1 / genetics
  • HIV-1 / immunology*
  • Immunization
  • Neutralization Tests
  • Protein Precursors / genetics
  • Protein Precursors / immunology*
  • Protein Precursors / isolation & purification
  • Rabbits
  • Recombination, Genetic
  • Vaccinia virus / genetics

Substances

  • Gene Products, env
  • HIV Antibodies
  • HIV Envelope Protein gp160
  • Protein Precursors