Polyphenols of Rosa L. leaves extracts and their radical scavenging activity

Z Naturforsch C J Biosci. 2007 Jan-Feb;62(1-2):32-8. doi: 10.1515/znc-2007-1-206.


Antioxidant potential of Rosa L. leaves methanolic extracts was evaluated in vitro using a spectrophotometric method based on measuring the radical scavenging effect on 2,2-di-phenyl-1-picrylhydrazyl (DPPH) radicals. The contents of ellagic acid, quercetin and kaempferol in the extracts from leaves of seventeen rose species were determined using SPE-RP-HPLC methods. Additionally, total phenolic content was determined spectrophotometrically according to the Folin-Ciocalteu procedure and calculated as gallic acid equivalents (GAE). Remarkable high antioxidant activity and high total phenolic content (5.7% < GAE < 15.2%), large ellagic acid (EA) content from 9.37 to 19.42 mg/g of dry weight, a quercetin content ranging from 3.68 to 15.81 mg/g of dry weight and kaempferol content from 1.25 to 9.41mg/g of dry weight were found in rose leaves. Significant correlation between EA (r(2) = 0.6131), quercetin (r(2) = 0.5158), total phenolic content (r(2)= 0.8485) and antioxidant activity was observed. Basing on the studies conducted one may assume that the extracts of rose leaves are a rich source of natural antioxidants and could be used to prevent free-radical-induced deleterious effects.

MeSH terms

  • Antioxidants / pharmacology
  • Chromatography, High Pressure Liquid
  • Ellagic Acid / metabolism
  • Flavonoids / chemistry
  • Flavonoids / isolation & purification*
  • Flavonoids / pharmacology
  • Flavonols / metabolism
  • Free Radical Scavengers / pharmacology*
  • Phenols / chemistry
  • Phenols / isolation & purification*
  • Phenols / pharmacology
  • Plant Extracts / chemistry
  • Plant Extracts / isolation & purification
  • Plant Leaves / chemistry*
  • Polyphenols
  • Rosa / chemistry*
  • Spectrophotometry


  • Antioxidants
  • Flavonoids
  • Flavonols
  • Free Radical Scavengers
  • Phenols
  • Plant Extracts
  • Polyphenols
  • Ellagic Acid