Isolation and Characterization of a Carbofuran-Degrading Strain Novosphingobium Sp. FND-3

FEMS Microbiol Lett. 2007 Jun;271(2):207-13. doi: 10.1111/j.1574-6968.2007.00718.x. Epub 2007 Apr 10.

Abstract

A gram-negative Novosphingobium sp. strain FND-3 capable of degrading carbofuran was isolated and characterized. The carbofuran-degrading ability of strain FND-3 was investigated under various culture conditions. Strain FND-3 showed a high average carbofuran-degrading rate of 28.6 mg L(-1) h(-1) in mineral salts medium with 100 mg L(-1) carbofuran. GC/MS analysis pointed out the presence of several unknown metabolites. One hydrolyzate was identified as 2-hydroxy-3-(3-methypropan-2-ol) phenol via hydrolysis of carbofuran phenol. The appearance of another metabolite with M(+) of 180 m/z indicated that the hydroxylation of carbofuran occurred at the aromatic ring. One novel degrading product with M(+) of 239 m/z was identified as 2-hydroxy-3-(3-methylpropan-2-ol) benzene-N-methylcarbamate via hydrolyzing at the ether bond of furanyl ring of carbofuran. Strain FND-3 was also able to degrade other N-methylcarbamate pesticides.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Carbofuran / chemistry
  • Carbofuran / metabolism*
  • DNA, Bacterial / chemistry
  • DNA, Bacterial / genetics
  • Gas Chromatography-Mass Spectrometry
  • Models, Chemical
  • Molecular Sequence Data
  • Molecular Structure
  • Phylogeny
  • RNA, Ribosomal, 16S / genetics
  • Sequence Analysis, DNA
  • Sphingomonadaceae / genetics
  • Sphingomonadaceae / metabolism*

Substances

  • DNA, Bacterial
  • RNA, Ribosomal, 16S
  • Carbofuran

Associated data

  • GENBANK/DQ831000