The effect of chlorhexidine on dentin hybrid layers in vivo

Oper Dent. Mar-Apr 2007;32(2):107-11. doi: 10.2341/06-55.


This in vivo study evaluated by TEM the degradation of dentin hybrid layers in deep occlusal resin composite restorations. Caries-free premolars scheduled for extraction as part of orthodontic treatment were prepared, restored and evaluated after two and six months. The adhesive used was a single-bottle etch-and-rinse product (Single Bond Plus, 3M ESPE). Control group restorations were placed according to the manufacturer's instructions, while the experimental group received application of a 2% solution of chlorhexidine digluconate after etching. No degradation was observed in either group after two months. Slight degradation was found in the control group after six months, but none was observed in the experimental group. In vitro testing showed no significant difference in microtensile bond strength between the control and experimental adhesive protocols.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acid Etching, Dental
  • Adolescent
  • Anti-Infective Agents, Local / chemistry*
  • Bisphenol A-Glycidyl Methacrylate / chemistry
  • Chlorhexidine / analogs & derivatives*
  • Chlorhexidine / chemistry
  • Composite Resins / chemistry*
  • Dental Bonding*
  • Dental Cavity Preparation
  • Dental Restoration, Permanent*
  • Dentin / ultrastructure*
  • Dentin-Bonding Agents / chemistry
  • Follow-Up Studies
  • Humans
  • Materials Testing
  • Microscopy, Electron, Transmission
  • Stress, Mechanical
  • Tensile Strength


  • Anti-Infective Agents, Local
  • Composite Resins
  • Dentin-Bonding Agents
  • Single Bond Plus
  • Bisphenol A-Glycidyl Methacrylate
  • chlorhexidine gluconate
  • Chlorhexidine