A major zebrafish polymorphism resource for genetic mapping

Genome Biol. 2007;8(4):R55. doi: 10.1186/gb-2007-8-4-r55.


We have identified 645,088 candidate polymorphisms in zebrafish and observe a single nucleotide polymorphism (SNP) validation rate of 71% to 86%, improving with polymorphism confidence score. Variant sites are non-random, with an excess of specific novel T- and A-rich motifs. We positioned half of the polymorphisms on zebrafish genetic and physical maps as a resource for positional cloning. We further demonstrate bulked segregant analysis using the anchored SNPs as a method for high-throughput genetic mapping in zebrafish.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Chromosome Mapping / methods*
  • Cloning, Molecular
  • Genetic Linkage
  • Genetic Markers
  • Polymorphism, Single Nucleotide*
  • Zebrafish / genetics*


  • Genetic Markers