Abstract
Penicillin resistance identification tests are important in veterinary medicine. Six enzyme assays and a PCR test were compared for the detection of beta-lactamase production or the beta-lactamase gene in 175 staphylococcal isolates. We conclude that the PCR test and two nitrocefin-based assays can be recommended for routine clinical use.
Publication types
-
Comparative Study
-
Evaluation Study
MeSH terms
-
Animals
-
Cattle
-
Cattle Diseases / microbiology
-
Cephalosporins / pharmacology*
-
Coagulase / metabolism
-
Colorimetry / methods
-
Indicators and Reagents / pharmacology*
-
Mastitis, Bovine / microbiology*
-
Microbial Sensitivity Tests
-
Polymerase Chain Reaction / methods*
-
Sensitivity and Specificity
-
Staphylococcal Infections / microbiology
-
Staphylococcal Infections / veterinary
-
Staphylococcus / drug effects
-
Staphylococcus / enzymology*
-
Staphylococcus / genetics
-
Staphylococcus / isolation & purification
-
Staphylococcus aureus / classification
-
Staphylococcus aureus / enzymology
-
Staphylococcus aureus / genetics
-
Staphylococcus aureus / isolation & purification
-
beta-Lactamases / biosynthesis*
-
beta-Lactamases / genetics
Substances
-
Cephalosporins
-
Coagulase
-
Indicators and Reagents
-
beta-Lactamases
-
nitrocefin