Purification, characterization, gene cloning, and expression of a novel alcohol dehydrogenase with anti-prelog stereospecificity from Candida parapsilosis

Appl Environ Microbiol. 2007 Jun;73(11):3759-64. doi: 10.1128/AEM.02185-06. Epub 2007 Apr 13.

Abstract

An alcohol dehydrogenase from Candida parapsilosis CCTCC M203011 was characterized along with its biochemical activity and structural gene. The amino acid sequence shows similarity to those of the short-chain dehydrogenase/reductases but no overall identity to known proteins. This enzyme with unusual stereospecificity catalyzes an anti-Prelog reduction of 2-hydroxyacetophenone to (S)-1-phenyl-1,2-ethanediol.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alcohol Dehydrogenase / genetics*
  • Alcohol Dehydrogenase / metabolism*
  • Amino Acid Sequence
  • Candida / enzymology*
  • Candida / genetics
  • Candida / metabolism
  • Cloning, Molecular
  • DNA, Fungal / chemistry
  • DNA, Fungal / genetics
  • Hydrogen-Ion Concentration
  • Molecular Sequence Data
  • Sequence Alignment
  • Sequence Analysis, DNA
  • Stereoisomerism
  • Substrate Specificity

Substances

  • DNA, Fungal
  • Alcohol Dehydrogenase

Associated data

  • GENBANK/DQ675534