Abstract
An alcohol dehydrogenase from Candida parapsilosis CCTCC M203011 was characterized along with its biochemical activity and structural gene. The amino acid sequence shows similarity to those of the short-chain dehydrogenase/reductases but no overall identity to known proteins. This enzyme with unusual stereospecificity catalyzes an anti-Prelog reduction of 2-hydroxyacetophenone to (S)-1-phenyl-1,2-ethanediol.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Alcohol Dehydrogenase / genetics*
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Alcohol Dehydrogenase / metabolism*
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Amino Acid Sequence
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Candida / enzymology*
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Candida / genetics
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Candida / metabolism
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Cloning, Molecular
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DNA, Fungal / chemistry
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DNA, Fungal / genetics
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Hydrogen-Ion Concentration
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Molecular Sequence Data
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Sequence Alignment
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Sequence Analysis, DNA
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Stereoisomerism
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Substrate Specificity
Substances
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DNA, Fungal
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Alcohol Dehydrogenase