A split zinc-finger protein is required for normal yeast growth

Gene. 1991 Oct 30;107(1):101-10. doi: 10.1016/0378-1119(91)90302-r.

Abstract

We have identified a gene that, when present in multiple copies, partially inhibits nuclear protein localization in Saccharomyces cerevisiae. This gene encodes a protein that is a unique member of the Cys2His2 zinc-finger family of DNA-binding proteins. It is designated SFP1 for split finger protein because its two zinc-finger domains are separated from one another by 40 amino acids (aa) as opposed to the usual spacing of 7 or 8 aa for Cys2His2 proteins. Disruption of the SFP1 gene results in slow cell growth, with cells having multiple, nucleated buds.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Blotting, Northern
  • Cloning, Molecular
  • DNA-Binding Proteins / genetics*
  • Fluorescent Antibody Technique
  • Fungal Proteins / analysis
  • Fungal Proteins / genetics
  • Gene Expression / genetics
  • Genes, Fungal / genetics*
  • Molecular Sequence Data
  • Nuclear Proteins / analysis
  • Recombinant Fusion Proteins / genetics
  • Restriction Mapping
  • Saccharomyces cerevisiae / genetics
  • Saccharomyces cerevisiae / growth & development*
  • Saccharomyces cerevisiae Proteins*
  • Zinc Fingers / genetics*

Substances

  • DNA-Binding Proteins
  • Fungal Proteins
  • Nuclear Proteins
  • Recombinant Fusion Proteins
  • SFP1 protein, S cerevisiae
  • Saccharomyces cerevisiae Proteins

Associated data

  • GENBANK/M59726
  • GENBANK/M59727
  • GENBANK/M59728
  • GENBANK/M63577
  • GENBANK/S66195
  • GENBANK/S66202
  • GENBANK/S66205
  • GENBANK/S66209
  • GENBANK/S66212
  • GENBANK/S68557