Effects of aestivation and starvation on the neutral lipid and phospholipid content of Biomphalaria glabrata infected with Schistosoma mansoni

J Parasitol. 2007 Feb;93(1):1-3. doi: 10.1645/GE-945R.1.

Abstract

The effects of aestivation or starvation on the neutral lipid and phospholipid content of Biomphalaria glabrata patently infected with Schistosoma mansoni were determined by high-performance thin-layer chromatography-densitometry. Infected-aestivated snails were maintained in a moist chamber at 24 +/- 1 C and a relative humidity of 98 +/- 1%. Infected-starved snails were maintained in artificial spring water (ASW) at 23 +/- 1 C without exogenous food. Infected snails (the controls) were maintained in ASW at 23 +/- 1 C and fed lettuce ad libitum. The 3 groups were maintained in the laboratory for 7 days, and then the lipids from the digestive gland-gonad complex (DGG) were extracted and analyzed by class. Infected-aestivated snails exhibited greater mortality rate and weight loss after 7 days than did the infected-starved snails. The steryl ester concentration in the infected-starved snails was significantly increased (P = 0.010) compared with the controls but not compared with infected-aestivated snails; the concentration of phosphatidylcholine in infected-aestivated snails was significantly decreased (P = 0.007) compared with the controls but not when compared with the infected-starved snails. Aestivation or starvation had a significant effect on the concentration of certain lipid classes in the DGG of B. glabrata infected with S. mansoni.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Biomphalaria / chemistry
  • Biomphalaria / parasitology*
  • Biomphalaria / physiology
  • Chromatography, High Pressure Liquid
  • Densitometry
  • Esters
  • Estivation / physiology*
  • Food Deprivation / physiology*
  • Lipids / analysis*
  • Phosphatidylcholines / analysis
  • Phospholipids / analysis
  • Schistosoma mansoni / physiology*
  • Sterols / analysis

Substances

  • Esters
  • Lipids
  • Phosphatidylcholines
  • Phospholipids
  • Sterols