Effect of propolis on survival of periodontal ligament cells: new storage media for avulsed teeth

J Endod. 2007 May;33(5):570-3. doi: 10.1016/j.joen.2006.12.021. Epub 2007 Feb 23.


Propolis is a multifunctional material used by bees in the construction and maintenance of their hives. Propolis possesses several biologic activities such as anti-inflammatory, antibacterial, antioxidant, antifungal, antiviral, and tissue regenerative, among others. The purpose of this study was to determine the ability of propolis to serve as a temporary storage medium for the maintenance of periodontal ligament (PDL) cell viability of avulsed teeth. PDL cells were obtained from healthy third molars and cultured in Dulbecco's Modified Eagles Medium (DMEM). Cultures were subjected to 10% propolis solution, 20% propolis solution, long-shelf life light milk with lower fat content (milk), Hank's Balanced Salt Solution, tap water as the negative control, and DMEM as the positive control. Tissue culture plates were incubated with experimental media at 37 degrees C for 1, 3, 6, 12, or 24 hours. PDL cell viability was assessed by trypan blue exclusion. Statistical analysis of the data was accomplished by using one-way analysis of variance complemented by the Tukey test. The level of significance was 5% (p<0.05). The results showed that 10% propolis was a more effective storage medium than other groups. In conclusion, propolis can be recommended as a suitable transport medium for avulsed teeth.

Publication types

  • Randomized Controlled Trial

MeSH terms

  • Analysis of Variance
  • Animals
  • Cell Survival / drug effects
  • Humans
  • Isotonic Solutions / therapeutic use
  • Milk
  • Organ Preservation Solutions / therapeutic use*
  • Periodontal Ligament / cytology
  • Periodontal Ligament / drug effects*
  • Propolis / therapeutic use*
  • Tissue Preservation / methods*
  • Tooth Avulsion / therapy


  • Hanks Balanced Salt Solution
  • Isotonic Solutions
  • Organ Preservation Solutions
  • Propolis