A simple, no-wash cell adhesion-based high-throughput assay for the discovery of small-molecule regulators of the integrin CD11b/CD18

J Biomol Screen. 2007 Apr;12(3):406-17. doi: 10.1177/1087057106299162.


The leukocyte-specific integrin CD11b/CD18 plays a key role in the biological function of these cells and represents a validated therapeutic target for inflammatory diseases. Currently, the low affinity interaction between CD11b/CD18 integrin and its respective ligand poses a challenge in the development of cell-based adhesion assays for the high-throughput screening (HTS) environment. Here the authors describe a simple cell-based adhesion assay that can be readily used for HTS for the discovery of functional regulators of CD11b/CD18. The assay consistently produces acceptable Z' values (> 0.5) for HTS. After testing the assay using 2 established blocking antibodies as reference biologicals, the authors performed a proof-of-concept primary screen using a library of 6612 compounds and identified both agonist and antagonist hits.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Antibodies, Blocking / pharmacology
  • CD11b Antigen / metabolism*
  • CD18 Antigens / metabolism*
  • Cell Adhesion / drug effects
  • Clone Cells
  • Dimerization
  • Drug Evaluation, Preclinical / methods*
  • Humans
  • K562 Cells
  • Molecular Weight


  • Antibodies, Blocking
  • CD11b Antigen
  • CD18 Antigens