Brucella Requires a Functional Type IV Secretion System to Elicit Innate Immune Responses in Mice

Cell Microbiol. 2007 Jul;9(7):1851-69. doi: 10.1111/j.1462-5822.2007.00922.x. Epub 2007 Apr 17.


The virB operon, encoding a Type IV secretion system (T4SS), is essential for intracellular survival and persistent infection by Brucella spp. To better understand the role of the T4SS in evading host defence mechanisms and establishing chronic infection, we compared transcriptional profiles of the host response to infection with wild-type and virB mutant Brucella strains. Analysis of gene expression profiles in murine splenocytes 3 days after inoculation with wild-type Brucella strains revealed an inflammatory response, with a prominent upregulation of genes induced by both type I and type II interferons. Real-time RT-PCR showed that a group of genes from these pathways were induced by day 3 post infection and declined to baseline levels by day 7. In contrast, neither of the two virB mutant strains elicited a proinflammatory gene expression profile, demonstrating that the T4SS was required to trigger this response. Infection studies using type I interferon receptor knockout mice showed that a lack of type I interferon signalling did not affect Brucella replication during the first 4 weeks of infection. Thus, induction of type I interferons does not appear to be an essential mechanism by which the T4SS promotes persistent infection by Brucella.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bacterial Proteins / immunology*
  • Bacterial Proteins / metabolism
  • Brucella abortus / immunology
  • Brucella abortus / pathogenicity*
  • Brucella melitensis / immunology
  • Brucella melitensis / pathogenicity
  • Brucellosis / immunology*
  • Brucellosis / microbiology
  • Female
  • Gene Expression Profiling
  • Gene Expression Regulation*
  • Immunity, Innate*
  • Interferons / genetics
  • Interferons / metabolism
  • Mice
  • Mice, Inbred C57BL
  • Molecular Sequence Data
  • Oligonucleotide Array Sequence Analysis
  • Proteins / genetics
  • Proteins / metabolism*
  • Spleen / cytology
  • Spleen / immunology


  • Bacterial Proteins
  • Proteins
  • Interferons

Associated data

  • GEO/GSE6810