Association between oxidative stress in pregnancy and preterm premature rupture of membranes

Clin Biochem. 2007 Jul;40(11):793-7. doi: 10.1016/j.clinbiochem.2007.03.004. Epub 2007 Mar 20.


Background: Premature rupture of membranes (PROM) is caused by collagen damage in the chorioamniotic sac leading to tearing. Reactive oxygen species (ROS) may be the cause of collagen damage. Isoprostanes (F(2)-IP) are produced by ROS attack on polyunsaturated fatty acids and are sensitive and specific biomarkers of lipid-peroxidation in vivo.

Aim: To verify whether oxidative stress occurs in pregnancies associated with preterm PROM.

Methods: F(2)-IPs were measured in amniotic fluid of 16 pregnancies with preterm PROM (Group II) and 97 without PROM (Group I).

Results: F(2)-IP concentrations (pg/mL) were significantly higher in group II than group I (p<0.0001). The ROC curve showed a sensitivity of 100% and a specificity of 84.5% at a cut-off of 124.4 pg/mL.

Conclusions: An association exists between oxidative stress in pregnancy and preterm PROM. The detection of amniotic fluid F(2)-IP concentrations seems to be a reliable predictive index of risk of preterm PROM.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Amniotic Fluid / metabolism
  • F2-Isoprostanes / metabolism
  • Female
  • Fetal Membranes, Premature Rupture / metabolism*
  • Fetal Membranes, Premature Rupture / pathology
  • Humans
  • Infant, Newborn
  • Oxidative Stress*
  • Pregnancy


  • F2-Isoprostanes