Complete identification of E-selectin ligands on neutrophils reveals distinct functions of PSGL-1, ESL-1, and CD44

Immunity. 2007 Apr;26(4):477-489. doi: 10.1016/j.immuni.2007.03.011.


The selectins and their ligands are required for leukocyte extravasation during inflammation. Several glycoproteins have been suggested to bind to E-selectin in vitro, but the complete identification of its physiological ligands has remained elusive. Here, we showed that E-selectin ligand-1 (ESL-1), P-selectin glycoprotein ligand-1 (PSGL-1), and CD44 encompassed all endothelial-selectin ligand activity on neutrophils by using gene- and RNA-targeted loss of function. PSGL-1 played a major role in the initial leukocyte capture, whereas ESL-1 was critical for converting initial tethers into steady slow rolling. CD44 controlled rolling velocity and mediated E-selectin-dependent redistribution of PSGL-1 and L-selectin to a major pole on slowly rolling leukocytes through p38 signaling. These results suggest distinct and dynamic contributions of these three glycoproteins in selectin-mediated neutrophil adhesion and signaling.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Adhesion
  • E-Selectin / immunology*
  • Hyaluronan Receptors / analysis
  • Hyaluronan Receptors / genetics
  • Hyaluronan Receptors / physiology*
  • Leukocyte Rolling / genetics
  • Ligands
  • Membrane Glycoproteins / analysis
  • Membrane Glycoproteins / genetics
  • Membrane Glycoproteins / physiology*
  • Mice
  • Mice, Knockout
  • Neutrophils / chemistry
  • Neutrophils / immunology*
  • Receptors, Fibroblast Growth Factor / analysis
  • Receptors, Fibroblast Growth Factor / genetics
  • Receptors, Fibroblast Growth Factor / physiology*
  • Sialoglycoproteins / analysis
  • Sialoglycoproteins / genetics
  • Sialoglycoproteins / physiology*


  • E-Selectin
  • Hyaluronan Receptors
  • Ligands
  • Membrane Glycoproteins
  • P-selectin ligand protein
  • Receptors, Fibroblast Growth Factor
  • Sialoglycoproteins
  • cysteine-rich fibroblast growth factor receptor