Comparison of the Mucosal Immune Response in Dogs Vaccinated With Either an Intranasal Avirulent Live Culture or a Subcutaneous Antigen Extract Vaccine of Bordetella Bronchiseptica

Vet Ther. Spring 2007;8(1):32-40.


Healthy dogs with low antibody titer to Bordetella bronchiseptica were vaccinated intranasally with an avirulent live vaccine, subcutaneously with an antigen extract vaccine, or subcutaneously and intranasally with a placebo. Intranasally vaccinated dogs developed B. bronchiseptica-specific IgA titers in nasal secretions that remained at high levels until the end of the study; dogs vaccinated subcutaneously with the antigen extract or placebo did not develop measurable antigen-specific IgA titers in nasal secretions. Dogs were challenged with virulent live B. bronchiseptica 63 days after vaccination. Intranasally vaccinated dogs had significantly lower cough scores (P < or =.0058) and shed significantly fewer challenge organisms (P <.0001) than dogs in either of the other groups. Cough scores of subcutaneously vaccinated dogs were not significantly different from placebo-vaccinated dogs.

Publication types

  • Randomized Controlled Trial
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Administration, Intranasal
  • Animals
  • Antibodies, Bacterial / blood
  • Antigens, Bacterial / immunology*
  • Bacterial Vaccines / administration & dosage
  • Bacterial Vaccines / therapeutic use*
  • Bordetella Infections / prevention & control
  • Bordetella Infections / veterinary*
  • Bordetella bronchiseptica / immunology*
  • Bronchitis / prevention & control
  • Bronchitis / veterinary*
  • Dog Diseases / immunology
  • Dog Diseases / prevention & control*
  • Dogs
  • Immunoglobulin A / metabolism
  • Injections, Subcutaneous / veterinary
  • Nasal Mucosa / immunology
  • Treatment Outcome
  • Vaccination / veterinary


  • Antibodies, Bacterial
  • Antigens, Bacterial
  • Bacterial Vaccines
  • Immunoglobulin A